![]() Increasing osmolality to 400–450 mOsm/kg did not result in an obvious change in viable cell density and product titer. To elucidate the effect of high osmolality and/or pCO2 on the production phase, experiments were conducted in bench‐scale bioreactors to more closely reflect the pCO2 and osmolality levels observed at large scale. This osmolality shift from 316 to 445 mOsm/kg resulted in an increase in specific production rates of lactate and ammonia by 43% and 48%, respectively. In contrast, increasing osmolality resulted in a linear reduction in specific cell growth rate (0.008 h−1/100 mOsm/kg) and led to a 60% decrease at 450 mOsm/kg as compared to the control at 316 mOsm/kg. Elevating pCO2 from 50 to 150 mmHg under controlled osmolality (about 350 mOsm/kg) resulted in a 9% reduction in specific cell growth rate. To understand the sole impact of pCO2 or osmolality on CHO cell growth, experiments were performed in bench‐scale bioreactors allowing one variable to change while controlling the other. pCO2 and osmolality, when elevated to high levels in bioreactors, can adversely affect cell culture and recombinant protein production. ![]() ![]() Partial pressure of CO2 (pCO2) and osmolality as high as 150 mmHg and 440 mOsm/kg, respectively, were observed in large‐scale CHO cell culture producing an antibody‐fusion protein, B1. Effects of Elevated pCO 2 and Osmolality on Growth of CHO Cells and Production of Antibody‐Fusion Protein B1: A Case Study Effects of Elevated pCO 2 and Osmolality on Growth of CHO Cells and Production of Antibody‐Fusion. ![]()
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